【GDNF】重組人神經(jīng)膠質(zhì)源神經(jīng)營養(yǎng)因子
產(chǎn)品報價
¥800/2μg ¥2080/10μg ¥74880/1mg
背景資料
GDNF promotes the survival and differentiation of dopaminergic neurons in culture, and is able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. In addition to the transcript encoding GDNF, two additional alternative transcripts encoding distinct proteins, referred to as astrocyte-derived trophic factors, have also been described. Mutations in this gene may be associated with Hirschsprung disease.
GDNF enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
產(chǎn)品描述
人神經(jīng)膠質(zhì)源神經(jīng)營養(yǎng)因子重組蛋白是在大腸桿菌中重組表達的,是由兩條非糖基化多肽鏈組成的同源二聚體。該重組蛋白包含有2 x 135個氨基酸,總分子量為30,360Da。該人神經(jīng)膠質(zhì)源神經(jīng)營養(yǎng)因子重組蛋白最終由標準的色譜技術(shù)純化出。
來源宿主
大腸桿菌中重組表達
序列
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Pro-Asp-Lys.
溶解建議
提供的人神經(jīng)膠質(zhì)源神經(jīng)營養(yǎng)因子 (GDNF)產(chǎn)品為過濾滅菌的白色凍干粉末;該凍干粉末是從含人神經(jīng)膠質(zhì)源神經(jīng)營養(yǎng)因子 (GDNF)的10mM檸檬酸鈉和150mM NaCl溶液中經(jīng)透析并凍干產(chǎn)生的。我們建議加無菌的18MΩ-cm水以配制工作儲存液到100μg/ml,后繼實驗過程中可根據(jù)實驗要求進一步稀釋。
保存建議
人神經(jīng)膠質(zhì)源神經(jīng)營養(yǎng)因子 (GDNF)凍干粉末在室溫可保存3周,或置于-18℃凍存用于長期保存。重新溶解后可以在4℃冷藏保存至少2-7天以上;如果想延長保存時間,建議分裝(加載體蛋白如0.1% HSA或 BSA)后置于-18℃凍存。請避免反復凍融。
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